Extended Data Fig. 1: Synthesis strategy for antibody-sensor conjugates for biodistribution of antibody and GzmB cleavage.

a, Synthetic scheme of antibody-sensor conjugate labelled with near infrared fluorophore VT750 to determine antibody biodistribution. b, Synthetic scheme of NIR activatable GzmB probe, consisting of fluorescently quenched GzmB substrate conjugated to αPD1 antibody.