Extended Data Fig. 2: Biodistribution of antibody-sensor conjugates and GzmB cleavage. | Nature Biomedical Engineering

Extended Data Fig. 2: Biodistribution of antibody-sensor conjugates and GzmB cleavage.

From: Urinary detection of early responses to checkpoint blockade and of resistance to it via protease-cleaved antibody-conjugated sensors

Extended Data Fig. 2

a, Schematic of antibody-sensor conjugate labelled with near infrared fluorophore VT750 to determine antibody biodistribution. b, Representative whole organ images and c, quantification of distribution of fluorescent signal in tumour and major organs (dLNs, tumour draining lymph nodes) (two-way ANOVA with Sidak’s post-test and correction for multiple comparisons, ns = not significant, n = 5 biological replicates, error bars depict s.e.m.). d, Schematic and in vitro cleavage of NIR activatable GzmB probe labelled with quenched near infrared fluorophore 800CW to localize site of probe activation (FC, fold change; ****P < 0.0001, n = 3 technically independent wells, error bars depict s.e.m.). e, Representative whole organ images and f, quantification of reporter fluorescence after treatment with Iso-GS or αPD1-GS (two-way ANOVA with Sidak’s post-test and correction for multiple comparisons, *P = 0.040, n = 4-5 biological replicates, error bars depict s.e.m.).

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