Extended Data Fig. 3: Single-cell RNA-seq profiling of murine organoid differentiation with KPT-330-mediated XPO1 inhibition.
a, Single-cell RNA-seq quality metrics on a per-sample basis, including final cell number (barcodes) per array, and distributions of unique molecular identifiers per barcode (UMI), unique gene number per barcode, and percent of total UMIs corresponding to mitochondrial genes per barcode. b, Single-cell RNA-seq quality metrics on a per-cell type basis, including final cell number (barcodes) per array, and distributions of unique molecular identifiers per barcode (UMI), unique gene number per barcode, and percent of total UMIs corresponding to mitochondrial genes per barcode. c, Feature plots over organoid differentiation UMAP representing module scores derived from gene sets enriched in in vivo stem cells, enterocytes, goblet cells, Paneth cells, and enteroendocrine cells, each score scaled on a range from 0 to 1. d, Feature plots over organoid differentiation UMAP restricted to stem I / II / III populations representing module scores derived from gene sets enriched in in vivo type I / II / III intestinal stem cells (ISCs), each score scaled on a range from 0 to 1. e, Violin plots for stem I / II / III populations representing module scores derived from gene sets enriched in in vivo type I / II / III intestinal stem cells (ISCs), each score scaled on a range from 0 to 1. Effect size measured as Cohen’s d, $ 0.5 < d < 0.8, $$$ 1.2 < d < 2, $$$$ d > 2. f, Organoid differentiation UMAP labeled by annotated cell type, and split by day 0 (ENR + CV), day 0.25–6 control (ENR + CD), and day 0.25–6 160 nM KPT-330-treated (ENR + CD + KPT-330).
