Extended Data Fig. 4: CISD1-Binding-labelled mitochondria begin to fuse with lysosomes after 4-cell stages.
From: Reduction of mtDNA heteroplasmy in mitochondrial replacement therapy by inducing forced mitophagy
Mouse embryos were microinjected with the CISD1-Binding mRNA at the zygote stage and stained with Blue DND-22 to label lysosomes at 1-cell, 2-cell and 4-cell stages before imaging. The insets indicate that CISD1-Binding-labeled mitochondria fuse with lysosomes (orange only). Bar = 30 μm. Also, see Fig. 3b and e. Three independently repeated biological experiments with similar results.
