Extended Data Fig. 5: CISD1-RFP-Binding(mutation)-labelled mitochondria do not recruit LC3 proteins and fuse with lysosomes in mouse embryos.
From: Reduction of mtDNA heteroplasmy in mitochondrial replacement therapy by inducing forced mitophagy
a. Mouse zygotes were microinjected with the mRNA encoding CISD1-RFP-Binding(mutation) and GFP-LC3. The insets indicate that the CISD1-RFP-Binding(mutation)-labelled mitochondria do not recruit GFP-LC3 in mouse embryos. b. Mouse zygotes were microinjected with the mRNA encoding CISD1-RFP-Binding(mutation) and stained with Blue DND-22 to label lysosomes before imaging. The insets indicate no fusion between the CISD1-RFP-Binding(mutation)-labelled mitochondria and lysosomes in mouse embryos. In a and b, scale bars, 30 μm, and experiments with similar results were biologically repeated twice.
