Extended Data Fig. 2: CISD1-Binding expresses immediately and localizes to mitochondria after its mRNA microinjection in mouse oocytes and Zygotes.
From: Reduction of mtDNA heteroplasmy in mitochondrial replacement therapy by inducing forced mitophagy
The mRNA encoding CISD1-Binding was microinjected into mouse GV oocytes, MII oocytes, and early pronuclear embryos. Images were obtained 1.5 hours after microinjection. Red indicates CISD1-Binding proteins and Green indicates MitoTracker Deep Red FM. Pearson’s correlation coefficient assessed co-localization between the CISD1-Binding and Mitotracker. Data were analyzed from three independently repeated experiments, and each point represents one cell. n = 10 for GV oocytes; n = 11 for MII oocytes and n = 12 for zygotes. The mean ± SEM values are 0.899 ± 0.01286 (GV), 0.9491 ± 0.00667 (MII) and 0.9467 ± 0.0076 (Zygote). Bar = 30 μm.
