Extended Data Fig. 6: Flow-cytometric quantification of major immune cell populations and macrophage polarized states in D5 wounds.
From: A strain-programmed patch for the healing of diabetic wounds
a-m, Single-cell suspensions were generated from excised wound and peri-wound tissues and stained for the indicated cell surface proteins. Percentage of immune cells (a), neutrophils (b), monocytes (c), macrophages (d), dendritic cells (DCs) (e), and T-cells (f) and T-cell subsets (g,h). Percentage of macrophages expressing markers CD86 (i), CD80 (j), CD163 (k), CD206 (l), and CD301b (m). Each data point represents pooled cells from two mice (four wounds). Values represent the mean and the standard deviation (n = 6 for TD Only, 7 for No Strain and 7 for Strain; independent samples). Statistical significance and p values are determined by one-way ANOVA followed by Fisher’s least significant difference (LSD) post-hoc test; ns, not significant.
