Extended Data Fig. 9: Immunostaining analysis of diabetic in vivo wound healing of porcine skin.

a,b, Representative immunohistochemistry images for CD31 (a) and quantification of CD31 + vessels per unit area (b) on day 7 (D7). c,d, Representative immunohistochemistry images for CD31 (c) and quantification of CD31 + vessels per unit area (d) on day 14 (D14). e,f, Representative immunofluorescence images for αSMA (e) and quantification of αSMA + cells per unit area (f) on D7. g, Representative immunofluorescence images for αSMA on D14. In immunohistochemistry images, the NOVA Red peroxidase substrate chromogenic stain was used. In immunofluorescence images, blue fluorescence corresponds to cell nuclei stained with 4′,6-diamidino-2-phenylindole (DAPI); green fluorescence corresponds to the expression of αSMA. Experimental groups are Tegaderm (TD) only, no strain (\(\lambda _{{{{\mathrm{patch}}}}}^{{{{\mathrm{pre}}}}} = 1\)) and strain-programmed (\(\lambda _{{{{\mathrm{patch}}}}}^{{{{\mathrm{pre}}}}} = 1.3\)) patch for both D7 and D14. Values in b,d,f represent the mean and the standard deviation (n = 6; independent samples). Statistical significance and p values are determined by one-way ANOVA followed by Fisher’s least significant difference (LSD) post-hoc test; ns, not significant. Scale bars, 100 µm (a,c,e,g).