Extended Data Fig. 10: Accelerated diabetic in vivo wound healing of humanized mouse skin.

a, Schematic illustrations for the xenotransplantation procedure, diabetes induction, and experimental plan. W, week. b, Representative images from Day 5 wounds with Masson’s trichrome stain (MTS). Red triangles denote wound margins. c-g, Quantification of the wound closure expressed as % of open wound compared to Day 0 (c), the re-epithelialization expressed as % (d), the hyperproliferative epidermis (HPE) area (e), the number of CD31 + vessels per unit area (f), and the number of αSMA + cells per unit area (g) on Day 5. Values in c-g represent the mean and the standard deviation (n = 4 in c, f, g; n = 4 for No Strain and 3 for Strain in d and e; independent samples). Statistical significance and p values are determined by two-sided t-test. Scale bars, 5 mm (a); 250 µm (b). Parts of (a) were created with BioRender.com.