Extended Data Fig. 8: Ex vivo whole brain imaging using vMSOT and mesoSPIM with LOCs h-FTAA and q-FTAA.
From: Multiscale optical and optoacoustic imaging of amyloid-β deposits in mice
a, 3D rendering of an anatomical vMSOT image (acquired at 600 nm) overlayed with unmixed AOI987 distribution rendered by vMSOT in NTL and arcAβ mice. b, 3D rendering of mesoSPIM data of Aβ distribution (stained with LCOs h-FTAA and q-FTAA) in NTL and arcAβ mice. c, Quantification of regional fluorescence intensity in the arcAβ mouse brain. Cortex: Ctx; Hippocampus: Hip; Midbrain: MB; Striatum: Str; Thalamus: TH. d,e, Sagittal views of the brain of 24 month-old non-transgenic littermate (NTL) (d) and arcAβ mouse (e). High Aβ plaque load was observed in the cortex, hippocampus and thalamus, while fewer plaques were observed in cerebellar and other subcortical regions. Fluorescence intensity (F.I.). Scale bar = 1 mm.
