Extended Data Fig. 5: Investigation of the effect of the number of C-Ag(I)-C artificial base pairs in TEprobes on the response of MARVE towards target RNA.
a, Absorbance at 560 nm in the presence of 1 nM target RNA and absence of target RNA (upper), and the ratio of background (in the absence of target RNA) to signal (in the presence of 1 nM target RNA) using TEprobes with 1, 2 and 3 C-Ag(I)-C artificial base pairs (bottom). b, Sequences of TEprobes with 1, 2 or 3 C-Ag(I)-C artificial base pairs. c, Evaluation of the discrimination capacity of TEprobes that anchored multiple C-Ag(I) ion-C artificial base pairs. The TEprobes were designed with forward toehold length ranged from 9 nt to 15 nt. The reverse toehold length of all TEprobes was 7 nt. Data in a and c are mean ± s.d. (n = 3). Concentrations of urea, phenol red, urease and TEprobes were 500 mM, 250 μM, 1 nM and 3 nM, respectively. The measurement of absorbance was proceeded after 20-min urea cleavage reaction.
