Fig. 6: Detection of SARS-CoV-2 and its mutations in clinical throat swab samples by MARVE.
a, Accumulated GARs of tested genes and mutations for each throat swab sample. D614G-mutated, Alpha, Beta and Gamma variants were covered in the detection using MARVE. b, Comparison of detection results by MARVE and RT–qPCR. Ct value of RT–qPCR is indicated as grey circles. Normalized GAR is shown in histogram. Normalized GAR is defined as (GARgene – GARnegative)/(GARRNase P – GARnegative), where GARgene, GARnegative and GARRNase P are GARs of the detection sites for target viral genes or mutations, the negative control and the human RNase P gene, respectively. Thresholds for Ct and normalized GAR values for positive and negative tests (red dashed lines) were obtained on the basis of ROC curves. Samples 1–15 were PCR-negative and 16–50 were PCR-positive. c, ROC accuracy of MARVE for SARS-CoV-2 diagnosis by targeting the N gene or the E gene. d, Detection results of SARS-CoV-2 and its mutations in 50 throat swab samples using MARVE.
