Extended Data Fig. 2: Changes in chromatin condensation with substrate stiffness in sparse chromatin or dense chromatin compartments. | Nature Biomedical Engineering

Extended Data Fig. 2: Changes in chromatin condensation with substrate stiffness in sparse chromatin or dense chromatin compartments.

From: Aberrant chromatin reorganization in cells from diseased fibrous connective tissue in response to altered chemomechanical cues

Extended Data Fig. 2

a,b, The Voronoi polygon density of the sparse (a) or dense (b) chromatin compartment is shown normalized to the Voronoi polygon density in human tenocytes grown on Glass, showing a decrease/increase in the condensation of dense chromatin compartment on stiff/soft substrates (n = 5 nuclei/group, *: p < 0.001 vs. glass, +: p < 0.001 vs. soft, one-way ANOVA). Changes in chromatin condensation in sparse chromatin (c) or dense chromatin (d) compartments. The Voronoi polygon density of the sparse or dense chromatin compartment is shown normalized to the Voronoi polygon density in human young tenocytes grown under normoxic (N) conditions, showing an increase in the condensation of dense chromatin under hypoxic (H) conditions (n = 5 nuclei/group, *: p < 0.001 vs. young healthy tenocyte under the normoxic conditions, +: p < 0.001 vs. tendinosis tenocyte under the normoxic conditions, one-way ANOVA). Changes in chromatin condensation with exposure to inflammatory cytokines in sparse chromatin (e) or dense chromatin (f) compartments. The Voronoi polygon density of the sparse or dense chromatin compartment is shown normalized to the Voronoi polygon density in human young tenocytes without treatment with inflammatory cytokines, showing an increase in the condensation of dense chromatin upon treatment (n = 5 nuclei/group, *: p < 0.001 vs. young human tenocyte control, +: p < 0.001 vs. tendinosis young human tenocyte, One-way ANOVA). Error bars, means ± s.d.

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