Extended Data Fig. 1: Custom-PDMS microfluidic chamber, and changes in chromatin distribution.
(a) Picture and schematic of a custom-PDMS microfluidic fluid shear stress (FSS) device. (b) Representative STORM super-resolution images of H2B rendered as a density map showing redistribution of H2B in hMSCs in response to FSS of varying magnitude 1 ~ 5 dyne/cm2) and duration (0.5 ~ 2 h). (c) Quantification of changes in the ratio of the total number of H2B localizations per unit area at the nuclear border to the total number of H2B localizations at the inner part of the nucleus with/without the application of FSS (*: p < 0.05 vs. Ctrl, +: p < 0.05 vs. 1D/0.5 h, a: p < 0.05 vs. 5D/0.5 h). The values have been normalized to Ctrl cells. (d) Changes in chromatin condensation with the application of FSS in (d) sparse chromatin compartment or (e) dense chromatin compartment in hMSCs. The Voronoi polygon density of the dense or the sparse chromatin compartment in cells subjected to FSS is shown normalized to the Voronoi polygon density in the absence of FSS (n = 5 nuclei/group, *: p < 0.001 vs. Ctrl, +: p < 0.001 vs. 1D/0.5 h, a: p < 0.001 vs. 5D/0.5 h, one-way ANOVA). Experiments were carried out at least in duplicate. Error bars, means ± s.d.
