Fig. 4: Base editing activity window summary of size-minimized ABEs in HEK293T cells. | Nature Biomedical Engineering

Fig. 4: Base editing activity window summary of size-minimized ABEs in HEK293T cells.

From: Efficient in vivo base editing via single adeno-associated viruses with size-optimized genomes encoding compact adenine base editors

Fig. 4

a–c, Base editing activity windows from 8–9 genomic target sites for Nme2ABE8e (a), CjABE8e (b) and SauriABE8e (c). Positions that were not present in any tested site are shaded grey. Target adenines are numbered with respect to a standard protospacer length for each editor (24 nt for Nme2ABE8e, 22 nt for CjABE8e and 21 nt for SauriABE8e). Dots represent values and error bars represent mean ± s.e.m. of n = 3 biological replicates for each site, with each position representing 1–3 genomic sites. The dotted line corresponds to 25% of mean peak activity for each base editor and defines the activity threshold considered within the editing window. Individual sites that contained three or more adenines within a protospacer were included in this analysis. Sites with only one or two adenines within the protospacer were excluded from summary analysis, but data from all sites analysed are shown in Fig. 3. d, The percentage of genomic adenines in the hg38 human reference genome targetable by size-minimized ABEs, either independently or collectively. An example showing a representative portion of the human genome targetable by size-minimized ABEs is shown, with targetable PAMs denoted with coloured lines and targetable adenines denoted with coloured dots.

Back to article page