Extended Data Fig. 5: Transduction of oligodendrocytes, motor neurons and peripheral tissues by AAV.CPP.16 and AAV9 in BALB/cJ. | Nature Biomedical Engineering

Extended Data Fig. 5: Transduction of oligodendrocytes, motor neurons and peripheral tissues by AAV.CPP.16 and AAV9 in BALB/cJ.

From: Variants of the adeno-associated virus serotype 9 with enhanced penetration of the blood–brain barrier in rodents and primates

Extended Data Fig. 5

a-e, Transduced oligodendrocytes (Olig2+) in the brain and quantification on percentage. Scale bar for a, b: 25 μm. Scale bar for c, d: 10 μm. n = 3 mice per group, mean ± s.e.m. f, Immunostaining of spinal cord sections. Motor neurons were visualized using ChAT antibody. Scale bars: 250 μm for the left two and 50 μm for the right two. g, Transduction of peripheral tissues and quantification on relative fold change. BALB/cJ mice were examined 3 weeks after intravenous injection of 1× 1012 vg AAV.CPP.16-CAG-H2B-GFP or AAV9-CAG-H2B-GFP. Mean ± s.e.m., n = 4-8, Student’s t-test.

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