Extended Data Fig. 6: Imaging, western blot and histological analysis for AAV.CPP.16-aPD-L1-treated GBM-bearing mice. | Nature Biomedical Engineering

Extended Data Fig. 6: Imaging, western blot and histological analysis for AAV.CPP.16-aPD-L1-treated GBM-bearing mice.

From: Variants of the adeno-associated virus serotype 9 with enhanced penetration of the blood–brain barrier in rodents and primates

Extended Data Fig. 6

a, Timeline for procedures in a mouse glioblastoma (GBM) model. b, Example of bioluminescence imaging for verification of tumor formation at day 7 post tumor implantation. c, aPD-L1-HA expression in the liver and muscle as measured by western blot. d-f, Representative images of hematoxylin-eosin-stained brain sections in a long-term survivor mouse treated with AAV.CPP.16-aPD-L1. Little residual tumor tissue was detectable suggesting eradication of GBM. g, Immunostaining showing AAV.CPP.16-mediated expression of aPD-L1-HA and astrogliosis in one long-term-surviving animal. Scale bar in g: 25 μm. n = 3-5 mice per group, mean ± s.e.m., one-way ANOVA with Tukey’s post-test.

Source data

Back to article page