Extended Data Fig. 4: CLIP integrates Cas13d into both ACTB and IL2RG, but is more highly expressed in ACTB.
From: Stable expression of large transgenes via the knock-in of an integrase-deficient lentivirus
a, Expression of Cas13d in either the ACTB or IL2RG locus in K562s (2 biological replicates with technical duplicates). b, Apparent knock-in efficiency is lower in IL2RG than ACTB (left) and the magnitude of expression of Cas13 is also lower in IL2RG than ACTB (right) (****: p < 0.0001) c, gDNA from K562s that underwent Cas13d CLIP in both loci was isolated. PCR was performed using a forward primer targeting Cas13d and reverse targeting IL2RG downstream of homology arm (left, expected band: 1101 bp) or using a forward primer targeting Cas13d and reverse targeting ACTB downstream of homology arm (right, expected band: 1059 bp). Data is from two biological replicates each with two technical replicates.
