Extended Data Fig. 1: CLIP’s cut sites enable more efficient HDR than IDLV.
From: Stable expression of large transgenes via the knock-in of an integrase-deficient lentivirus
CLIP strategy compared to IDLV (same as CLIP but without inclusion of cut sites) with and without RNP 3, 5, and 7 days after electroporation in K562s. The high expression peak is indicative of a targeted knock-in while the low expression peak is indicative of transient episomal expression of the reverse transcribed viral genome. Data from two biological replicates each with technical duplicates (replicate 1 shown in Fig. 2c).
