Extended Data Fig. 2: Additional controls for the FACS analyses of anti-MUC16 antibodies.
From: Computational optimization of antibody humanness and stability by systematic energy-based ranking
a, Non-transfected HEK293 cells were stained with a commercial anti-tag antibody, cαMUC16_Ab1, hαMUC16_Ab1, cαMUC16_Ab2, hαMUC16_Ab2.1, and hαMUC16_Ab2.2, demonstrating that none of the five antibodies showed significant binding to cells not expressing the MUC16 construct. b, HEK293 cells expressing the MUC16 construct were stained either without a primary antibody or with a commercial anti-tag antibody, demonstrating that the secondary antibody alone does not label the cells and that the cells do display the intended construct. c, Acetone-fixed OVCAR-3 cells were stained either without a primary antibody or with a control anti-MUC16 antibody69, demonstrating that the secondary antibody alone does not label the cells and that the cells do display MUC16.
