Fig. 1: Conjugation of l-Ser to butyrate maintained its biological activity. | Nature Biomedical Engineering

Fig. 1: Conjugation of l-Ser to butyrate maintained its biological activity.

From: A serine-conjugated butyrate prodrug with high oral bioavailability suppresses autoimmune arthritis and neuroinflammation in mice

Fig. 1

a, Chemical synthesis of serine conjugate with butyrate (SerBut). TFA, trifluoroacetic acid. RT, room temperature. b, Whole cell lysates of Raw 264.7 macrophages stimulated with the indicated concentrations of NaBut, SerBut or trichostatin A (TSA) as well as 100 ng ml−1 LPS for 18 h were probed for histone acetylation activity via western blot. c, Experimental schema on BMDCs incubated with NaBut or SerBut at a series of concentrations for 24 h, followed by LPS stimulation for 18 h. d, Percentage of live BMDCs after treatment. e–g, Percentage of MHC class II+ (e), CD80+ (f) or CD86+ (g) BMDCs analysed by flow cytometry. h, TNF concentration in the cell culture supernatant of BMDCs. n = 3; data are representative of two independent experiments. Data represent mean ± s.e.m. Statistical analyses were performed using one-way ANOVA with Dunnett’s test. P values less than 0.05 are shown. Panel c created with BioRender.com.

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