Extended Data Fig. 4: Immunological effects of SerBut treatment in healthy C57BL/6 mice on the T cells in the spleen, LNs, or lamina propria. | Nature Biomedical Engineering

Extended Data Fig. 4: Immunological effects of SerBut treatment in healthy C57BL/6 mice on the T cells in the spleen, LNs, or lamina propria.

From: A serine-conjugated butyrate prodrug with high oral bioavailability suppresses autoimmune arthritis and neuroinflammation in mice

Extended Data Fig. 4

a. Experimental schema. C57BL/6 mice at 10 wks old were oral administered with PBS (n = 5), NaBut (15 mg, molar equivalent to SerBut, n = 6) or SerBut (25 mg, n = 6), twice daily for 10 days, and sacrificed for cellular analysis. b. CD4+Foxp3+ Tregs percentage of live cells in spleen, mesenteric LNs (mLNs), hock-draining LNs (hock-dLNs), and spinal cord-draining LNs (SC-dLNs). c. Tbet+, GATA3+, RORγ+ Foxp3−, RORγ+ Foxp3+, PD-1+, CTLA-4+ of CD4+ T cells in the spleen. Data represent mean ± s.e.m. Statistical analyses were performed using one-way ANOVA with Tukey’s post hoc test. P values less than 0.05 were shown.

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