Extended Data Fig. 1: Varying hydrophobicity in the engineered polypeptide affects immunogenicity via ER stress-mediated mtDNA release and effector functions in macrophages.
a, Chemical structure of cationic polypeptides with different amine-containing analogues. Cationic polypeptides including hydrophilic analogues and cyclic structures more favorably induced. (b) ER stress and (c) mtDNA release in BMDMs (n = 3). The representative western blot images were shown from at least twice independent results. Cationic polypeptide tethered with a hydrophilic building block and cyclic structure increased (d) phagocytosis of EO771 breast cancer cells and (e) cross-presentation of the model antigen SIINFEKL-H2Kb (n = 3). (f) Gene expression of pro-inflammatory cytokines was affected by hydrophobicity of polypeptides and the chemical structure of amine-including analogues (n = 3). One-way ANOVAs with Bonferroni post hoc correction were used in c, d, e, f. All data are expressed as means±s.d.
