Extended Data Fig. 6: Statistical validation of SOMM’s capability to detect calcium events across a 50-250 µm imaging depth range.

a. Example traces from 5 cortical depths were recorded by hybrid “temporally interleaved” 2p and SOMM microscope, where 2p signals are blue and SOMM signals are red. For each modality, calcium transient events were identified by OASIS (vertical lines). Seven traces are randomly selected for each cortical depth. Each recording was 100 s long. Scale bar: 10 seconds. Neurons are randomly ordered. b. Calcium event detection scores (precision, sensitivity, and F-score) achieved by SOMM on experimental functional verification dataset as a function of depth. Mean ± SD of precision scores are 0.78 ± 0.27, 0.73 ± 0.31, 0.69 ± 0.33, 0.65 ± 0.35, 0.54 ± 0.31 from 50 to 250 μm imaging depth at a step of 50 μm. Mean ± SD of sensitivity scores are 0.74 ± 0.27, 0.68 ± 0.27, 0.67 ± 0.28, 0.54 ± 0.29, 0.51 ± 0.31 from 50 to 300 μm im-aging depth at a step of 50 μm. Mean ± SD of F scores are 0.71 ± 0.24, 0.65 ± 0.26, 0.62 ± 0.27, 0.52 ± 0.26, 0.50 ± 0.24 from 50 to 250 μm imaging depth at a step of 50 μm. n = 1479 neurons across 7 imaging sessions from 2 mice. c. Linear fits to the normalized peak intensity of each neuron (blue cross) as a function of calcium transient detection F score. The solid red line represents the fitted model and the dashed red line represents the 95% confidence bounds. n = 1479 neurons across 7 imaging sessions from 2 mice. d is the same as c but shows linear fits to the standard deviation of normalized temporal activity of each neuron as a function of the calcium transient detection F score. n = 1479 neurons across 7 imaging sessions from 2 mice.