Extended Data Fig. 3: Cell-ratio-based meta-analyses.
a, Normalized permeability values of bi-culture (pericyte and EC) BBB models versus ratio groups of pericyte (X) to EC (1). Each ratio group represents a range of pericyte (X) to EC ratios (1). b, Normalized permeability values of bi-culture (astrocyte and EC) BBB models versus ratio groups of astrocyte (X) to EC (1). Each ratio group represents a range of astrocyte (X) to EC ratios (1). c, Normalized permeability values of tri-culture (pericyte, astrocyte, and EC) BBB models versus ratio groups of pericyte and astrocyte altogether (X) to EC (1). Each ratio group represents a range of pericyte and astrocyte altogether (X) to EC ratios (1). d, Normalized permeability values of tri-culture (pericyte, astrocyte, and EC) BBB models versus ratio groups of astrocyte (X) to pericyte (1) from these models, with respect to (that is, normalized by) the corresponding EC mono-culture models. For a-c, statistical analyses were performed using one-way ANOVA with post hoc Tukey’s multiple comparisons tests. There is no statistical significance between any of the groups. In b, the dark color-filled, solid circle represents an outlier which was not included in the statistical analysis. In each panel, every unfilled, hollow circle represents a different, non-outlier data set of the listed model/co-culture. For data sets with n < 3, the violin was omitted and only the data points were plotted. For the violin plots in a-c, the coarsely-dashed, thicker line in each violin represents the median (50th percentile) of the data set, and the upper and lower more finely-dashed, thinner lines represent the upper (75th percentile) and lower (25th percentile) quartiles of the data set, respectively. In d, a line of best fit is shown together with the line equation and goodness of fit (R2). NP and NA refer to the number of pericyte (P) and astrocyte (A), respectively. The ranges of NP > NA and NA > NP are shaded in different colors in d.
