Extended Data Fig. 3: Structural characterization of distinct organoid phenotypes from epithelial and mesenchymal PDAC subtypes.
a Phalloidin (red)/DAPI (blue) IF staining (top) of the distinct epithelial and mesenchymal organoid phenotypes. Surface segmentation of the above images with Imaris Scale bars= 300 μm for TEBBO, cystic branched, 500 μm for thick branched and tree-like, 400 μm for the branched mesenchymal and 200 μm for the firework and star-like organoids. b-j Morphological parameters analysis for epithelial (from the mouse line ID: 9591) organoid phenotypes: TEBBO (n = 106 organoids), cystic branched (n = 105 organoids), thick branched (n = 267 organoids) and tree-like (n = 57 organoids). b Major axis length of epithelial organoids in μm. c Thickness of core branches in μm. d Violin plot of average number of main branches. e Total number of nodes. f Average number of spiky branches. g Average number of terminal end buds. h Number of swollen lumens/microlumens i Lumen size of the epithelial organoid phenotypes measured in μm2. b-c and e-i Graphs represent median with interquartile range. Unpaired two-tailed non parametric t-test, Mann-Whitney test. j % average level of granular appearance of organoids (low, medium, high). k-n Morphological parameters analysis for mesenchymal (from the mouse line ID: 16992) organoid phenotypes: branched mesenchymal (n = 158 organoids), firework (n = 261 organoids) and star-like (n = 206 organoids). k Major axis length of mesenchymal organoids in μm. l Branch (or core branch) thickness measured in μm. m Average number of total branches (main and subbranches). k-m Graphs represent median with interquartile range. Unpaired two-tailed non parametric t-test, Mann-Whitney test. n Comparison of the core area of star-like organoids to the theoretical perfect circular area by measuring the diameter (radius=diameter/2) of the organoids and then calculating the area (A), A= π*r2. Graph represents median with interquartile range.
