Extended Data Fig. 8: Multi-Switch receptor arrays enhance the activity of triple-CAR T cells.
From: Leucine zipper-based immunomagnetic purification of CAR T cells displaying multiple receptors
a, Diagrams and vector maps for triple-CAR ± dual-Switch receptor arrays. b, Flow cytometry analysis of Zip-sorted triple-CAR and triple-CAR dual-Switch albino B6 T cells. Numbers in flow plots represent post-sort purity percentage for each construct. c, 24 h luciferase-based target lysis assay with Zip-sorted BAFF-R/CD79b/CD20 triple-CAR BALB/c T cells with targets as depicted. Data are mean ± SEM of triplicate wells. d–f, Sublethally irradiated albino B6 mice were injected with 1:1:1 ratio of C1498-CD20, C1498-CD79b, C1498-BAFF-R and treated with dasatinib-cultured triple-CAR ± dual-Switch T cells co-transduced with a membrane-bound Gaussia luciferase (gLuc) vector: gLuc-PD-1H-CD24-GPI-P2A-EGFP for T cell BLI. d, C1498 BLI (CBR). e, Survival; from a single experiment. f, T cell BLI (Gaussia). Data are mean ± SEM of biological replicates. g,h, Experimental setup as in panels d–f, but with stress test 0.4 × 106 T cell dose and T cell BLI not performed. g, C1498 BLI from two combined experiments (left) and images from representative experiment (right). h, Survival. Log-transformed BLI AUC values were compared using a Vardi test with FDR correction. Survival differences were compared via pairwise log-rank test, with FDR correction.
