Extended Data Fig. 9: Quad-CAR T cells including a single-domain antibody-based CD19-CAR demonstrate cognate target lysis, but exhibit limited antileukaemia activity in vivo.
From: Leucine zipper-based immunomagnetic purification of CAR T cells displaying multiple receptors
a, Diagram and vector maps for quad-CAR receptor array. b, Flow cytometry analysis of CAR expression. c, 24 h luciferase-based target lysis assay with quad-CAR B6 T cells and targets as listed. Data are mean ± SEM of triplicate wells for representative experiments from n = 2 donors. d–g, Sublethally irradiated albino B6 mice were injected with 1:1:1:1 ratio of C1498 singly expressing (hCD19, CD20, CD79bΔ, BAFF-R) and treated with dasatinib-cultured quad-CAR albino B6 T cells co-transduced with Gaussia luciferase vector gLuc-PD-1H-CD24-GPI-P2A-EGFP. d, C1498 BLI from three combined experiments with different BLI imaging timing. BLI AUC values were compared using a Vardi test with FDR correction. e, Survival. Differences in survival were compared with a log-rank test. f, Representative day 20 T cell BLI (Gaussia) and day 21 C1498 BLI (CBR) images. g, Antigen expression analysis from C1498 harvested from bone marrow of mice reaching humane endpoints. Percentage hCD19+ C1498 was compared with two-tailed t-test. Data are mean ± SEM of biological replicates.
