Extended Data Fig. 3: Schematic representation of the two-bead assay performed on 55 pre-pandemic and 39 COVID-19 samples. | Nature Biomedical Engineering

Extended Data Fig. 3: Schematic representation of the two-bead assay performed on 55 pre-pandemic and 39 COVID-19 samples.

From: High-throughput multiplexed serology via the mass-spectrometric analysis of isotopically barcoded beads

Extended Data Fig. 3

a, Each well of 10 plates with 924 sample IDs contained 20 Target ID barcodes that were utilized to recognize beads loaded with SARS-CoV-2 Spike S1, Spike S1 RBD, Spike S2, Nucleocapsid, or non-loaded negative control (“Empty”). Each target had four barcodes in this experiment. b, For each serum sample, 100 nL, 10 nL, and 1 nL were tested. Each dilution was done in triplicate, and for each replicate each target was tested four times for 12 replicates per sample. Pre-pandemic samples (negative control) are shown in green and COVID-19-positive samples are shown in red.

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