Fig. 7: FUS-MB treatment with fixed PNP and repeat sonications can prevent de novo lesion formation.
a,c,e, Top rows: T1-weighted spin echo images taken immediately following FUS-MB treatment with hyperintense signal denoting the focal column. Middle and bottom rows: minimum intensity projection images of longitudinal T2-weighted spin echo images to visualize through 1 mm of the focal column for representative mice in the (a) single sonication with fixed PNP arm, (c) repeat sonication with fixed PNP arm or (e) repeat sonication with PCD-modulated PNP arm. Black ovals denote contralateral non-sonicated ROIs for de novo quantification, while coloured ovals represent sonicated ROIs. b,d,f, Paired line graphs comparing the change in CCM number 1 month following FUS-MB between the sonicated brain region and the contralateral non-sonicated brain region for mice in the (b) single sonication with fixed PNP arm (n = 6 CCM mice), (d) repeat sonication with fixed PNP arm (n = 6 CCM mice) or (f) repeat sonication with PCD-modulated PNP arm (n = 4 CCM mice). Concentric circles indicate multiple mice with the same number of de novo CCMs. Colours indicate applied PNP. For mice receiving the repeat FUS regimen with fixed PNP, the number of new lesions formed in the sonicated brain region is significantly reduced compared with the contralateral brain region. *P = 0.0312; two-tailed Wilcoxon matched-pairs signed-rank test. g, Plot of de novo CCM formation and PNP for all treatment conditions. **P = 0.0064; linear regression indicates that de novo CCM formation and PNP are inversely correlated. h, Plot of de novo CCM formation and number of FUS applications (that is, # of Tx) for all treatment conditions. P = 0.0914; linear regression indicates that de novo CCM formation and # of Tx are not inversely correlated.
