Extended Data Fig. 10: LF fascicle Young’s modulus significantly decreases after 24 hour incubation with Ripasudil, and decreases significantly more than when incubated with DMSO.
A, Timeline describing experimental protocol for LF collection from the operating room through beginning incubation with DMSO or ripasudil, referred to in graphs as ‘0 h’, and competition of the incubation period, referred to in graphs as ‘24 h’. B,C, Schematics demonstrating the hypothesized components resisting tension and thus contributing to LF fascicle bulk modulus - ECM fibers and cellular cytoskeletal tension, the latter of which would be disrupted with Ripasudil treatment. D, In 6 sets of quadruply-paired fascicles from 5 patients, the Young’s modulus of fascicles treated with Ripasudil decreased significantly (p = 0.0012) while the Young’s modulus of fascicles treated with DMSO decreased non-significantly (p = 0.0523). E, Fascicles treated with Ripasudil had a significantly greater negative change in modulus (YMend - YMstart) than fascicles treated with DMSO (p = 0.0467). Tests are paired, one-tailed t-tests.
