Extended Data Fig. 1: Lack of cross reaction between Bama pig SIRPα and human CD47 and rejection of human cells by Bama pig macrophages in vivo.

(a) Biotin conjugated human CD47-fc was incubated with human or Bama pig PBMCs followed by streptavidin-PE staining and examined by flow cytometry. The controls were stained with streptavidin-PE alone. Shown are representative profiles showing hCD47-fc staining of SSC^high monocytes/macrophages. (b, c) Human and pig RBCs were labeled with high or low intensity of CFSE respectively, mixed at 1:1 ratio and intravenously injected into Bama pigs that were pre-treated with GdCl3 to deplete macrophages (n = 1) or saline (as control; n = 1). Flow cytometric profiles (b) and levels (%; c) of human RBCs (CFSE^hi) in total surviving CFSE⁺ cells are shown.