Extended Data Fig. 7: Flow cytometric analysis of EMT6 tumors following two doses of AP-diABZI.

EMT6 tumor bearing female Balb/c mice were treated with two intravenous doses of AP-diABZI (n = 8) or PBS (n = 7) and tumors isolated 24 h later for flow cytometric analysis of (a) frequency of live and Ki67⁺ breast cancer cells (CD45^-), (b) frequency of NK cells, CD8⁺ T cells, and CD4⁺ T cells expressing the indicated markers in EMT6 tumors, and (c) frequency of NK cells, CD8⁺ T cells, and CD4⁺ T cells expressing the indicated markers in the spleen. (d) Frequency of Ki67⁺CD69⁺ and Ki67⁺PD-1⁺ CD8⁺ T cells and CD4⁺ T cells in the spleens of mice treated as indicated. P values determined by two-tailed Student’s t-test. Replicates are biological, and data are shown as mean ± SEM.