Extended Data Fig. 9: Effect of NK and T cell depletion on AP-diABZI efficacy in B16.F10 model. | Nature Biomedical Engineering

Extended Data Fig. 9: Effect of NK and T cell depletion on AP-diABZI efficacy in B16.F10 model.

From: Potentiating cancer immunotherapies with modular albumin-hitchhiking nanobody–STING agonist conjugates

Extended Data Fig. 9

(a) Schematic of B16.F10 tumor inoculation and treatment schedule with depletion antibodies (n = 9 for PBS, anti-CD4, and AP-diABZI; n = 8 for all other groups). Anti-NK1.1 IgG, anti-CD8 IgG, and anti-CD4 IgG were injected I.P. at 200 µg and AP-diABZI was injected I.V. at 1.25 µg of diABZI per injection. (b) Tumor growth curves and (c) Kaplan-Meier survival plots for mice with B16.F10 tumors treated as indicated. (b) P values determined by two-way ANOVA with post-hoc Tukey’s correction for multiple comparisons; comparisons to PBS on day 19 are shown. (c) Endpoint criteria of 1500 mm3 tumor volume with P value determined by log-rank test for comparison to PBS group and for the comparisons indicated. Replicates are biological, and data are shown as mean ± SEM. Panel a created with BioRender.com.

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