Extended Data Fig. 1: Design and fabrication of leukaemia bone marrow niche chip. | Nature Biomedical Engineering

Extended Data Fig. 1: Design and fabrication of leukaemia bone marrow niche chip.

From: Bioengineered immunocompetent preclinical trial-on-chip tool enables screening of CAR T cell therapy for leukaemia

Extended Data Fig. 1

(a) The actual chip of human leukaemia bone marrow where chambers and channels were filled with food dyes. (b) The scanning electron microscopy images of detailed design of the microfluidic chip. (c) The whole scanning of the leukaemia bone marrow niche on-chip, where RFP-expressing HUVCEs form a vascular network (red), hFOB1.19 osteoblast (yellow) and fibroblast establish an endosteal region, and Reh B-ALL cells (green) sparsely distribute throughout the niche. (d) Representative images showing the presence of hematopoietic stem cells (CD34+) in yellow in the endosteal niche. (e) Representative images showing the presence of B cell (CD19+ without GFP) and B-ALL blasts (CD19+ and GFP). (f) Representative images showing the presence of macrophages (CD68+) in yellow. (g) The 3D cross-section view of the stromal compartment. Vascular cells in green (CD31+), bone marrow stem/stromal cells in red (CD90+), leukaemia blasts in yellow (CD19+), and nuclei in blue (DAPI). (h) The 3D view of the vascular niche. Hematopoietic cells in red (CD45+), leukaemia blasts in green (CD19+), vessel in white (CD31+), and nuclei in blue (DAPI). Representative images were from one of three technical replicates with similar results (n = 3).

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