Extended Data Fig. 2: Cell self-assembling and tissue remodeling.
(a) Scanning electronic microscopy images of tissue-engineered chip after 7-day culture. The hollow structure of microvessel formed during on-chip culture (red dash line, left) and extracellular matrix where bone marrow cells are embedded (right). (b) Representative images showing 3D view of key ECM components (collagen IV, fibronectin, and laminin) deposited by bone marrow niche cells on-chip after 7-day culture. (c) The schematic showing cell self-assembling. (d) Representative images showing time-lapse ECM deposition by bone marrow cells during 7-day culture. Representative images in a, b, d were from one of three independent experiments with similar results (n = 3). (e) Quantification of ECM deposition. Collagen IV (top), Fibronectin (middle), and Laminin (bottom). Images were collected from five technical replicates (n = 5) and processed with CellProfiler (version 4.1.3). One-way ANOVA followed by Kruskal-Wallis’ test, mean and s.e.m.
