Extended Data Fig. 4: Recall antibody response post-challenge in mice vaccinated with inactivated virus coated on the floss.
From: Floss-based vaccination targets the gingival sulcus for mucosal and systemic immunization
Mice were immunized with either 10 µg or 25 µg of Inac. A/Puerto Rico/8/34 virus (PR8) influenza virus coated on floss. Mice either received two or three immunizations. UnVacc. mice were used as a negative control. On day 57, mice including the UnVacc. group were challenged with 3×LD50 of influenza A PR8 virus. On day 4 post-challenge, different mucosal secretions such as fecal pellets, vaginal wash, and saliva were collected from mice. Mice were then euthanized, and LL and NW were collected from euthanized mice. Anti-Inac. virus specific IgG and IgA in fecal pellets (a), vaginal wash (b), and saliva (c). Anti-Inac. virus specific IgG in LL (d), and NW (e) of immunized (floss) or UnVacc. mice. Data are presented as the arithmetic mean ± SD of samples from individual animals. N = 4 to 5 mice in each group. The Mann–Whitney Unpaired test (two-tailed) was used to calculate p values for differences between groups.
