Extended Data Fig. 5: Recall antibody response post-challenge in mice vaccinated with M2e-AuNP + CpG.
From: Floss-based vaccination targets the gingival sulcus for mucosal and systemic immunization
Mice were vaccinated on day 0 and day 21 with M2e-AuNP+CpG (1X) vaccine formulation either coated on floss or administered IN. UnVacc. mice were used as negative controls. On day 43, mice were challenged with 3×LD50 of influenza A H1N1pdm09 virus. On day 4 post-challenge, different mucosal secretions, such as vaginal wash and saliva, were collected from the mice. Mice were then euthanized, and LL and NW were collected from euthanized mice. Anti-M2e IgG and IgA in vaginal wash (a), and saliva (b), LL (c), and NW (d) of immunized (floss/ IN) or UnVacc. mice. N = 3 to 5 mice in each group. Data are presented as the arithmetic mean ± SD of samples from individual animals. The Mann–Whitney Unpaired test (two-tailed) was used to calculate p values for differences between groups.
