Fig. 4: Validation across diverse tissue sizes and types. | Nature Biomedical Engineering

Fig. 4: Validation across diverse tissue sizes and types.

From: Multiparametric cellular and spatial organization in cancer tissue lesions with a streamlined pipeline

Fig. 4

a, We tested small TMA cores, tissue biopsies and whole-slide tissue resections on MARQO, with these tissues tiled and segmented here. Scale bars, 50 µm. b, MARQO’s segmentation and classification performance after quality control by the user for markers CD3 and PanCK are shown for the following tissue sizes and types: whole-slide resected tissue NSCLC and TMA cores for NSCLC, head and neck squamous cell carcinoma (HNSCC), colorectal cancer (CRC), breast cancer (BC), epithelial ovarian cancer (EOC), pancreatic ductal adenocarcinoma (PDAC), glioblastoma (GBM), renal cell carcinoma (RCC) and melanoma (MEL). Scale bars, 50 µm. c, Box plots depicting MARQO densities and scatter plots with regression analysis lines comparing MARQO densities with QuPath-derived densities for the corresponding tissue sizes and types are shown here for markers CD3 and PanCK (n = 4 per tissue type). Box plots show the median (centre line), 25th and 75th percentiles (bounds of the box), and minimum and maximum values (whiskers). For the scatter plot, each plotted point corresponds to a biologically replicated resection sample or TMA core. Non-parametric Spearman’s correlation r values and linear regression analyses were used and associated P values are shown per tissue type per regression.

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