Fig. 5: Integrative platform for other staining technologies. | Nature Biomedical Engineering

Fig. 5: Integrative platform for other staining technologies.

From: Multiparametric cellular and spatial organization in cancer tissue lesions with a streamlined pipeline

Fig. 5

a, Singleplex IHC examples segmented and classified after quality control by the user for marker CD3 with chromogen DAB (top) and marker PDL1 with chromagen AEC (bottom). Scatter plots with non-parametric Spearman regression analysis compare MARQO densities with QuPath-derived densities for CD3 (top) and PDL1 (bottom). Spearman correlation r values with associated P values are shown per marker. Scale bars, 30 µm. b, mIF using COMET immunofluorescence technology to assess MARQO’s ability to segment and classify another multiplex technology after user quality control. We depict a representative tile with cellular nuclear stain DAPI and the 4 previous overlapping markers of interest (n = 4 per marker): FOXP3, CD3, CD68 and PanCK. Stemming from this tile (right) is the single DAPI stain, adjacent to the MARQO segmentation mask overlaid on the DAPI image. Also stemming from the tile (bottom) is each of the marker channels of interest above that marker channel with MARQO’s classification channel after user quality control overlaid. The top-right panel is a scatter plot with non-parametric Spearman regression analysis to compare MARQO densities with QuPath-derived densities for all four markers. Spearman correlation r values with associated P values are shown per marker. Scale bar, 30 µm.

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