Fig. 4: Development of bbCARMEN with biotinylated crRNA beads in equipment-free generated droplets.
From: Bead-based approaches for increased sensitivity and multiplexing of CRISPR diagnostics
a, Schematic of the bbCARMEN workflow. b, Merged fluorescence images of colour-coded crRNA beads in droplets. SARS-CoV-2: AF546 shown as yellow; RNase P: AF647 shown as blue; FAM reporter shown as green. c, Fluorescence from droplets from individual crRNA bead solutions and pooled bead solutions at 30 min post-reaction initiation. Individual points represent fluorescence from individual droplets. Median fluorescence values are plotted with 95% CIs. d, Fluorescence kinetics of amplified SARS-CoV-2 synthetic gene fragment at 106 copies per μl and RNase P in pooled crRNA bead solution from b and c. Red, SARS-CoV-2; dark grey, RNase P; light grey, NTC. Median fluorescence values are plotted with 95% CIs. e, Heat map showing SARS-CoV-2, RNase P and NTC median fluorescence values at 30 min post-reaction initiation in single and pooled crRNA bead solutions from b and c, conducted at 106 copies per μl.
