Fig. 5: Implementation of respiratory virus panel on bbCARMEN.
From: Bead-based approaches for increased sensitivity and multiplexing of CRISPR diagnostics
a, Schematic of multiplexed RVP assay with each of the 9 viruses on panel differentiated by distinct colour codes. b, Fluorescence across SARS-CoV-2 dilution series from 106–101 copies per μl and corresponding NTC fluorescence in a pooled bead solution containing all 10 RVP crRNAs. Green, SARS-CoV-2; grey, NTC. Line indicates median fluorescence 30 min post-reaction initiation. c, Fluorescence at 104 copies per μl for all 9 viruses on RVP. Fluorescence shown as the median value across 20 replicates at 30 min post-reaction initiation. *, expected reactivity based on crRNA sequence design. d, Scatterplot comparison of SARS-CoV-detection at 0-, 30- and 60-min timepoints using bbCARMEN and RT–qPCR. Ct values computed as means of three technical replicates. e, Concordance of RT–qPCR and NGS results compared to CARMEN v2. Right: RT–qPCR results. Left: NGS results. Positive versus negative ground truth as determined by three technical replicates for qPCR.
