Extended Data Fig. 1: Schematic illustration and flow cytometry validation of the library construction.
From: Systematic functional screening of switchable aptamer beacon probes
The monoclonal microbeads library of aptamer beacons screening was constructed by emulsion PCR amplification, restriction enzyme-enabled site-specific modification, and exonuclease-digested single-strand generation, which were tested on primer-immobilized microbeads using. The library comprises a randomized region (pink), forward primers (blue and yellow), and reverse primer binding sites (yellow and green). Intramolecular complementary regions (yellow) and restriction enzyme cutting sites (letters) are embedded within. Fluorophores and quenchers are both attached onto dU bases.
