Supplementary Figure 2: Caspase activation is not impacted by Nec1-mediated RIPK1 inhibition. | Nature Cell Biology

Supplementary Figure 2: Caspase activation is not impacted by Nec1-mediated RIPK1 inhibition.

From: RIPK1-mediated induction of mitophagy compromises the viability of extracellular-matrix-detached cells

Supplementary Figure 2

(a-f) BT474 (a), T47D (b), MCF7 (c), HeLa (d), HCT116 (e), and H460 (f) cells were grown in Att and Det after being treated with DMSO or Nec1 (10μM) for 48h. Caspase-3/7 activation was measured 48h after plating in Att and Det using the CaspaseGlo 3/7 assay. Results are presented as means +/- SEM and statistical significance was calculated using a Student’s two tailed t-test. Caspase activation is represented as a ratio of Det value over Att value. n=3 independent biological samples for each cell line. N.S., not significant. (g) 10A-Bcl2 cells were grown in soft agar and imaged each day for three days. Cells were scored as viable or necrotic as indicated. Results are presented as percentage of necrotic cells +/- SD. n=12 independent biological samples. (h) 10A-Bcl2 cells were grown in soft agar and imaged each day for three days. Cells were scored as viable or entotic as indicated. Results are presented as percentage of entotic cells +/- SD. n=12 independent biological replicates.

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