Supplementary Figure 2: Validation of iPSCs produced with Ncor1/2 knockdown. | Nature Cell Biology

Supplementary Figure 2: Validation of iPSCs produced with Ncor1/2 knockdown.

From: NCoR/SMRT co-repressors cooperate with c-MYC to create an epigenetic barrier to somatic cell reprogramming

Supplementary Figure 2

(a) Phase contrast and Oct4-GFP images and immunofluorescence staining for the indicated pluripotency markers (red) for iPSC clones derived from MEFs transduced with OSKM and shRNAs against Ncor1 or Ncor2 in serum+Vc (also for all subsequent panels). Scale bars, 100 μm (phase contrast and GFP fluorescence image) and 50 μm (immunofluorescence). (b) PCR showing integration of the exogenous transgenes in the genome of the indicated iPSC clones, OG2 ESCs were included as control. (c) RT-qPCR showing silencing of exogenous transgenes in the indicated iPSC clones. Data are the mean of 2 technical replicates from 1 experiment relative to MEFs transduced with OSKM on day 6. (d) RT-qPCR showing endogenous expression of the indicated pluripotency genes in the indicated iPSC clones. Data are the mean of 2 technical replicates from 1 experiment. (e) Karyotype analysis of the indicated iPSC clones. (f) Chimeric mice generated with the indicated iPSC clones.

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