Supplementary Figure 6: ATM levels and activity are diminished in old HSCs.
a-b) Expression of a) DNA damage response and b) DNA damage sensor genes in young and old HSCs, (n = 3–5 mice per group, as indicated with dots). Data represents normalized signal intensity (data from Gene Expression Commons4,43, Gene Expression Omnibus (Series GSE55525). c) Olive tail moment of young and old freshly isolated HSCs or upon 1 hour in culture post irradiation with 2Gy. Data represent 1 experiment, n numbers represent cells and are indicated in the Figure. d) Dot blot showing γH2AX and actin levels in LSKs non-treated or irradiated (1 hour), with and without ATM inhibitor (ATMi, 10 μM). Experiment was performed once. e) Quantification of γH2AX levels in c-kit enriched bone marrow upon irradiation and 4h of culture with or ATMi. n = 3 mice. *, P< 0.05, **, P< 0.005, ***, P< 0.0005 (Two-tailed Student’s t-test). Centre bar represents mean and error bars represent SEM. Source data are included in Supplementary Table 1.
