Supplementary Figure 4: TP53INP2 increases WNT signaling. | Nature Cell Biology

Supplementary Figure 4: TP53INP2 increases WNT signaling.

From: TP53INP2 regulates adiposity by activating β-catenin through autophagy-dependent sequestration of GSK3β

Supplementary Figure 4

a) TOP-Flash and FOP-Flash reporter activity in control and TP53INP2-overexpressing HEK293T cells, and effects of LiCl. Data are means ± SEM of n = 3 independent experiments. Statistical significance was calculated using one-way ANOVA followed by Tukey’s test (*P = 0.0002 indicates effects caused by TP53INP2 overexpression and #P < 0.0001 indicates effects caused by LiCl; b) TOP-Flash and FOP-Flash reporter activity in control HEK293T cells and cells overexpressing wild-type, L36A/L40A or E97K/D98K mutant forms of TP53INP2, in the presence or absence of LiCl. Data are means ± SEM of n = 3 independent experiments. Statistical significance was calculated using one-way ANOVA followed by Tukey’s test (*P < 0.0001 indicates effects caused by TP53INP2 overexpression, and #P < 0.0001, §P = 0.0061, &P = 0.0004 indicates effects caused by LiCl).

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