Supplementary Figure 1: TP53INP2 regulates adipogenesis.

Panels a to h: Control (C) and TP53INP2 knockdown (KD) 3T3-L1 cells. (a) TP53INP2 mRNA (n = 4 independent experiments) and protein (n = 3 independent experiments) expression. Data are mean ± SEM. Statistical significance was calculated using an unpaired two-tailed t-test (^*P < 0.0001); (b) Distribution of adipocyte cell area at day 8 of differentiation. Data are mean ± SEM. Statistical significance was calculated using two-way ANOVA (^*P = 0.0005); (c) Triglycerides content. Data are mean ± SEM. Statistical significance was calculated using an unpaired two-tailed t-test (^*P < 0.0001); Panels d and e: GLUT4 (d) and perilipin (e) protein quantification in adipocytes. Data are mean ± SEM. Statistical significance was calculated using an unpaired two-tailed t-test (GLUT4: ^*P = 0.0004 and Perilipin: ^*P < 0.0001); (f) Insulin-stimulated 2-deoxy-glucose uptake. Data are mean ± SEM (n = 6 independent experiments). Statistical significance was calculated using two-way ANOVA followed by Sidak’s test (^*P = 0.0080); Panels g and h: PPARγ (g) and C/EBPα (h) protein quantification during differentiation. Data are mean ± SEM. Statistical significance was calculated using two-way ANOVA followed by Sidak’s test (PPARγ: ^*P < 0.0001; C/EBPα: ^*P = 0.0001 and ^#P < 0.0001); Panels i to n: Control (-) and TP53INP2- (+) overexpressing 3T3-L1CAR∆1 cells. (i) TP53INP2 mRNA and protein expression. Data are mean ± SEM. Statistical significance was calculated using an unpaired two-tailed t-test (^*P < 0.0001); (j) Triglycerides content. Data are mean ± SEM. Statistical significance was calculated using an unpaired two-tailed t-test (^*P = 0.0034); Panels k and l: GLUT-4 (k) and perilipin (l) protein quantification in adipocytes. Data are mean ± SEM. Statistical significance was calculated using an unpaired two-tailed t-test (GLUT4: ^*P = 0.0125 and Perilipin: ^*P < 0.0496); Panels m and n: PPARγ (m) and C/EBPα (n) protein quantification during differentiation. Data are mean ± SEM. Statistical significance was calculated using two-way ANOVA followed by Sidak’s test (PPARγ: ^*P = 0.0049, ^#P = 0.0004 and ^§P < 0.0001; C/EBPα: ^*P = 0.0029 and ^#P = 0.0060); o) Control and TP53INP2-overexpressing human preadipocytes. PPARγ protein expression during human adipogenesis. Data are mean ± SEM. Statistical significance was calculated using two-way ANOVA followed by Sidak’s test (^*P = 0.0269, ^#P = 0.0207 and ^§P < 0.0473). All unprocessed blots are provided in Supplementary Figure 8.