Supplementary Figure 2: TP53INP2 loss-of-function promotes adipose hyperplasia.

Panels a, b and c: TP53INP2 mRNA levels in human subcutaneous and visceral adipose tissue are inversely proportional to BMI. The sample size of the study 1 is n = 24 samples of scWAT (a) and n = 38 samples of vWAT (b). In the study 2, the sample size is n = 35 samples of scWAT. Statistical significance was calculated using a Spearman correlation analysis (r = -0.439 and P = 0.0051 (a); r = -0.567 and P = 0.0002 (b); r = -0.348 and P = 0.04 (c); d) Experimental design for the generation of TP53INP2 KO^Ubc mice: two-month-old mice expressing CreTP53INP2^loxP/loxP and their non-expressing Cre-ERT2TP53INP2^loxP/loxP control littermates were fed a tamoxifen diet. Experimental data were collected 2 months after starting the tamoxifen diet; Panels e to j: Control and TP53INP2 KO^Ubc four-month-old mice. Data represent means ± SEM. (e) TP53INP2 mRNA levels in ingWAT (n = 4 control and n = 5 KO^Ubc mice) and vWAT (n = 5 control and n = 7 KO^Ubc mice). Statistical significance was calculated using an unpaired two-tailed t-test (^*P = 0.0002 and ^#P < 0.0001); (f) TP53INP2 protein expression in ingWAT (n = 3) and pgWAT (n = 3) depots; (g) TP53INP2 mRNA levels in other tissues (n = 3 samples for each tissue). Statistical significance was calculated using an unpaired two-tailed t-test (^*P = 0.0139; ^#P = 0.0031; ^§P ≤ 0.0001 and ^&P = 0.0004); Panels h and i: Tissues weigh of females (h) (Brain, kidney and heart: n = 10 control and n = 15 KO^Ubc mice, and Gastroc, Quads and Tib: n = 5 control and n = 9 KO^Ubc mice) and males (i) (n = 4 control and n = 7 KO^Ubc mice). Data represent means ± SEM. Statistical significance was calculated using an unpaired two-tailed t-test; j) Fsp27, Perilipin, Leptin, Resistin, LPL and VEGF mRNA levels in adipose tissue. Data represent means ± SEM (n = 4 control and n = 6 KO^Ubc mice). Statistical significance was calculated using an unpaired two-tailed t-test (^*P = 0.0113; ^#P = 0.0320; ^§P < 0.0271; ^&P = 0.0452; ^°P = 0.0465 and ⁺P < 0.0107). All unprocessed blots are provided in Supplementary Figure 8.