Supplementary Figure 6: Cell fate asymmetry requires myosin II and Rho kinase activity. | Nature Cell Biology

Supplementary Figure 6: Cell fate asymmetry requires myosin II and Rho kinase activity.

From: Counter-rotational cell flows drive morphological and cell fate asymmetries in mammalian hair follicles

Supplementary Figure 6

Shh-Cre driving mGFP marks anterior placode cell fates (green) while Sox9 marks posterior placode cell fates (magenta). Representative immunofluorescence images of germ placodes cultured from E15.5 in the presence of DMSO (left), blebbistatin (center), and Y-27632 (right) quantified in Figure S4D. Shh and Sox9 expressing cells fail to separate along the AP axis in the presence of myosin or Rho kinase inhibitors. Scale bar, 10µm. Anterior is to the left.

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